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gradient pcr instrument  (Eppendorf AG)


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    Structured Review

    Eppendorf AG gradient pcr instrument
    Gradient Pcr Instrument, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 201853 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gradient+pcr+instrument/Eppendorf/pmc12860781-20-15-21
    Average 99 stars, based on 201853 article reviews
    gradient pcr instrument - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Mass Spectrometry:

    Article Title: Genomic Characterization and Antimicrobial Resistance of Four Mcr-1 Escherichia coli Strains Isolated from Human and Environment Sources, Hainan Province, Tropical China
    Article Snippet: .. Mass spectrometer VITEK MS (France Biomerieux), polymyxin B drug sensitivity reagent (Kangtai Bio), Vitek2 automatic bacterial identification instrument (France Biomerieux), MacConkey medium (China Guangdong Huankai), serologic diagnostic serum for E. coli O157:H7 (Ningbo Tianrun, China), TanonTM nucleic acid dye (China Shanghai Tianneng), 2 × Taq PCR master mix (Beijing Tiangen, China), Gradient PCR instrument (Eppendorf, Germany), automatic digital gel image analysis system (Tanon-4100, Tianneng, China), constant temperature shaking table (Shanghai Zhichu Instrument Co., Ltd.), and prokaryotic genome NGS sequencing were sent to Megi Biological Company. ..

    Diagnostic Assay:

    Article Title: Genomic Characterization and Antimicrobial Resistance of Four Mcr-1 Escherichia coli Strains Isolated from Human and Environment Sources, Hainan Province, Tropical China
    Article Snippet: .. Mass spectrometer VITEK MS (France Biomerieux), polymyxin B drug sensitivity reagent (Kangtai Bio), Vitek2 automatic bacterial identification instrument (France Biomerieux), MacConkey medium (China Guangdong Huankai), serologic diagnostic serum for E. coli O157:H7 (Ningbo Tianrun, China), TanonTM nucleic acid dye (China Shanghai Tianneng), 2 × Taq PCR master mix (Beijing Tiangen, China), Gradient PCR instrument (Eppendorf, Germany), automatic digital gel image analysis system (Tanon-4100, Tianneng, China), constant temperature shaking table (Shanghai Zhichu Instrument Co., Ltd.), and prokaryotic genome NGS sequencing were sent to Megi Biological Company. ..

    Polymerase Chain Reaction:

    Article Title: Genomic Characterization and Antimicrobial Resistance of Four Mcr-1 Escherichia coli Strains Isolated from Human and Environment Sources, Hainan Province, Tropical China
    Article Snippet: .. Mass spectrometer VITEK MS (France Biomerieux), polymyxin B drug sensitivity reagent (Kangtai Bio), Vitek2 automatic bacterial identification instrument (France Biomerieux), MacConkey medium (China Guangdong Huankai), serologic diagnostic serum for E. coli O157:H7 (Ningbo Tianrun, China), TanonTM nucleic acid dye (China Shanghai Tianneng), 2 × Taq PCR master mix (Beijing Tiangen, China), Gradient PCR instrument (Eppendorf, Germany), automatic digital gel image analysis system (Tanon-4100, Tianneng, China), constant temperature shaking table (Shanghai Zhichu Instrument Co., Ltd.), and prokaryotic genome NGS sequencing were sent to Megi Biological Company. ..

    Article Title: Angiotensin II Induces Vascular Endothelial Dysfunction by Promoting Lipid Peroxidation-Mediated Ferroptosis via CD36.
    Article Snippet: The RNA of HUVECs was extracted by FreeZol Reagent (Vazyme, Cat. No. R71101, Nanjing, China). .. After the RNA concentration was detected, RNA was reversed to cDNA by Gradient PCR instrument (Eppendorf, Germany), and further qRT-PCR assays were performed by QuantStudioTM 6 Flex Real-Time PCR System (ABI, Los Angeles, CA, USA). ..

    Article Title: Dietary ellagic acid inhibiting gastrointestinal pathogens by modulation of microbiome-metabolite-immune axis.
    Article Snippet: For SCFAs analysis, a gas chromatography system (7890B GC System, Agilent Technologies, USA) coupled with a mass spectrometer (5977B GC/MSD, Agilent Technologies, USA) was employed. .. For PCR analysis, the following instruments were utilized: a spectrophotometer (BioPhotometer® D30, Eppendorf, Germany), a gradient PCR instrument (Mastercycler® nexus GX2, Eppendorf, Germany), and a LightCycler® 96 Instrument (Roche, Switzerland). ..

    Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis.
    Article Snippet: The extracted RNA was then reverse-transcribed into complementary DNA utilizing the HiScript III RT SuperMix for qPCR (+gDNA wiper) kit (TaKaRa). .. To assess the expression levels of individual genes, PCR amplification was conducted with Premix TaqTM in a Gradient PCR Instrument (Eppendorf, Germany). ..

    Article Title: Angiotensin II Induces Vascular Endothelial Dysfunction by Promoting Lipid Peroxidation-Mediated Ferroptosis via CD36
    Article Snippet: The RNA of HUVECs was extracted by FreeZol Reagent (Vazyme, Cat. No. R711-01, Nanjing, China). .. After the RNA concentration was detected, RNA was reversed to cDNA by Gradient PCR instrument (Eppendorf, Germany), and further qRT-PCR assays were performed by QuantStudio TM 6 Flex Real-Time PCR System (ABI, Los Angeles, CA, USA). ..

    Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis
    Article Snippet: The extracted RNA was then reverse-transcribed into complementary DNA utilizing the HiScript III RT SuperMix for qPCR (+ gDNA wiper) kit (TaKaRa). .. To assess the expression levels of individual genes, PCR amplification was conducted with Premix TaqTM in a Gradient PCR Instrument (Eppendorf, Germany). ..

    Article Title: Dietary ellagic acid inhibiting gastrointestinal pathogens by modulation of microbiome-metabolite-immune axis
    Article Snippet: For SCFAs analysis, a gas chromatography system (7890B GC System, Agilent Technologies, USA) coupled with a mass spectrometer (5977B GC/MSD, Agilent Technologies, USA) was employed. .. For PCR analysis, the following instruments were utilized: a spectrophotometer (BioPhotometer® D30, Eppendorf, Germany), a gradient PCR instrument (Mastercycler® nexus GX2, Eppendorf, Germany), and a LightCycler® 96 Instrument (Roche, Switzerland). ..

    Next-Generation Sequencing:

    Article Title: Genomic Characterization and Antimicrobial Resistance of Four Mcr-1 Escherichia coli Strains Isolated from Human and Environment Sources, Hainan Province, Tropical China
    Article Snippet: .. Mass spectrometer VITEK MS (France Biomerieux), polymyxin B drug sensitivity reagent (Kangtai Bio), Vitek2 automatic bacterial identification instrument (France Biomerieux), MacConkey medium (China Guangdong Huankai), serologic diagnostic serum for E. coli O157:H7 (Ningbo Tianrun, China), TanonTM nucleic acid dye (China Shanghai Tianneng), 2 × Taq PCR master mix (Beijing Tiangen, China), Gradient PCR instrument (Eppendorf, Germany), automatic digital gel image analysis system (Tanon-4100, Tianneng, China), constant temperature shaking table (Shanghai Zhichu Instrument Co., Ltd.), and prokaryotic genome NGS sequencing were sent to Megi Biological Company. ..

    Sequencing:

    Article Title: Genomic Characterization and Antimicrobial Resistance of Four Mcr-1 Escherichia coli Strains Isolated from Human and Environment Sources, Hainan Province, Tropical China
    Article Snippet: .. Mass spectrometer VITEK MS (France Biomerieux), polymyxin B drug sensitivity reagent (Kangtai Bio), Vitek2 automatic bacterial identification instrument (France Biomerieux), MacConkey medium (China Guangdong Huankai), serologic diagnostic serum for E. coli O157:H7 (Ningbo Tianrun, China), TanonTM nucleic acid dye (China Shanghai Tianneng), 2 × Taq PCR master mix (Beijing Tiangen, China), Gradient PCR instrument (Eppendorf, Germany), automatic digital gel image analysis system (Tanon-4100, Tianneng, China), constant temperature shaking table (Shanghai Zhichu Instrument Co., Ltd.), and prokaryotic genome NGS sequencing were sent to Megi Biological Company. ..

    Concentration Assay:

    Article Title: Angiotensin II Induces Vascular Endothelial Dysfunction by Promoting Lipid Peroxidation-Mediated Ferroptosis via CD36.
    Article Snippet: The RNA of HUVECs was extracted by FreeZol Reagent (Vazyme, Cat. No. R71101, Nanjing, China). .. After the RNA concentration was detected, RNA was reversed to cDNA by Gradient PCR instrument (Eppendorf, Germany), and further qRT-PCR assays were performed by QuantStudioTM 6 Flex Real-Time PCR System (ABI, Los Angeles, CA, USA). ..

    Article Title: Angiotensin II Induces Vascular Endothelial Dysfunction by Promoting Lipid Peroxidation-Mediated Ferroptosis via CD36
    Article Snippet: The RNA of HUVECs was extracted by FreeZol Reagent (Vazyme, Cat. No. R711-01, Nanjing, China). .. After the RNA concentration was detected, RNA was reversed to cDNA by Gradient PCR instrument (Eppendorf, Germany), and further qRT-PCR assays were performed by QuantStudio TM 6 Flex Real-Time PCR System (ABI, Los Angeles, CA, USA). ..

    Quantitative RT-PCR:

    Article Title: Angiotensin II Induces Vascular Endothelial Dysfunction by Promoting Lipid Peroxidation-Mediated Ferroptosis via CD36.
    Article Snippet: The RNA of HUVECs was extracted by FreeZol Reagent (Vazyme, Cat. No. R71101, Nanjing, China). .. After the RNA concentration was detected, RNA was reversed to cDNA by Gradient PCR instrument (Eppendorf, Germany), and further qRT-PCR assays were performed by QuantStudioTM 6 Flex Real-Time PCR System (ABI, Los Angeles, CA, USA). ..

    Article Title: Angiotensin II Induces Vascular Endothelial Dysfunction by Promoting Lipid Peroxidation-Mediated Ferroptosis via CD36
    Article Snippet: The RNA of HUVECs was extracted by FreeZol Reagent (Vazyme, Cat. No. R711-01, Nanjing, China). .. After the RNA concentration was detected, RNA was reversed to cDNA by Gradient PCR instrument (Eppendorf, Germany), and further qRT-PCR assays were performed by QuantStudio TM 6 Flex Real-Time PCR System (ABI, Los Angeles, CA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Angiotensin II Induces Vascular Endothelial Dysfunction by Promoting Lipid Peroxidation-Mediated Ferroptosis via CD36.
    Article Snippet: The RNA of HUVECs was extracted by FreeZol Reagent (Vazyme, Cat. No. R71101, Nanjing, China). .. After the RNA concentration was detected, RNA was reversed to cDNA by Gradient PCR instrument (Eppendorf, Germany), and further qRT-PCR assays were performed by QuantStudioTM 6 Flex Real-Time PCR System (ABI, Los Angeles, CA, USA). ..

    Article Title: Angiotensin II Induces Vascular Endothelial Dysfunction by Promoting Lipid Peroxidation-Mediated Ferroptosis via CD36
    Article Snippet: The RNA of HUVECs was extracted by FreeZol Reagent (Vazyme, Cat. No. R711-01, Nanjing, China). .. After the RNA concentration was detected, RNA was reversed to cDNA by Gradient PCR instrument (Eppendorf, Germany), and further qRT-PCR assays were performed by QuantStudio TM 6 Flex Real-Time PCR System (ABI, Los Angeles, CA, USA). ..

    Spectrophotometry:

    Article Title: Dietary ellagic acid inhibiting gastrointestinal pathogens by modulation of microbiome-metabolite-immune axis.
    Article Snippet: For SCFAs analysis, a gas chromatography system (7890B GC System, Agilent Technologies, USA) coupled with a mass spectrometer (5977B GC/MSD, Agilent Technologies, USA) was employed. .. For PCR analysis, the following instruments were utilized: a spectrophotometer (BioPhotometer® D30, Eppendorf, Germany), a gradient PCR instrument (Mastercycler® nexus GX2, Eppendorf, Germany), and a LightCycler® 96 Instrument (Roche, Switzerland). ..

    Article Title: Effects and Mechanisms of Cadmium on the Degradation of Microcystin-LR by Sphingopyxis sp. YF1
    Article Snippet: In this study, we investigated the effects of cadmium concentration on the degradation of microcystin-LR by Sphingopyxis sp. YF1, a highly efficient microcystins degrading bacterium.. Our results demonstrate that 0.. 25 mg/L cadmium decreased the degradation rate at 30 min, but had no significant effect on the biodegradation of MC-LR after 60 min; however, 4 mg/L cadmium significantly reduced the biodegradation rate of MC-LR throught the time points tested.

    Article Title: Dietary ellagic acid inhibiting gastrointestinal pathogens by modulation of microbiome-metabolite-immune axis
    Article Snippet: For SCFAs analysis, a gas chromatography system (7890B GC System, Agilent Technologies, USA) coupled with a mass spectrometer (5977B GC/MSD, Agilent Technologies, USA) was employed. .. For PCR analysis, the following instruments were utilized: a spectrophotometer (BioPhotometer® D30, Eppendorf, Germany), a gradient PCR instrument (Mastercycler® nexus GX2, Eppendorf, Germany), and a LightCycler® 96 Instrument (Roche, Switzerland). ..

    Expressing:

    Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis.
    Article Snippet: The extracted RNA was then reverse-transcribed into complementary DNA utilizing the HiScript III RT SuperMix for qPCR (+gDNA wiper) kit (TaKaRa). .. To assess the expression levels of individual genes, PCR amplification was conducted with Premix TaqTM in a Gradient PCR Instrument (Eppendorf, Germany). ..

    Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis
    Article Snippet: The extracted RNA was then reverse-transcribed into complementary DNA utilizing the HiScript III RT SuperMix for qPCR (+ gDNA wiper) kit (TaKaRa). .. To assess the expression levels of individual genes, PCR amplification was conducted with Premix TaqTM in a Gradient PCR Instrument (Eppendorf, Germany). ..

    Amplification:

    Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis.
    Article Snippet: The extracted RNA was then reverse-transcribed into complementary DNA utilizing the HiScript III RT SuperMix for qPCR (+gDNA wiper) kit (TaKaRa). .. To assess the expression levels of individual genes, PCR amplification was conducted with Premix TaqTM in a Gradient PCR Instrument (Eppendorf, Germany). ..

    Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis
    Article Snippet: The extracted RNA was then reverse-transcribed into complementary DNA utilizing the HiScript III RT SuperMix for qPCR (+ gDNA wiper) kit (TaKaRa). .. To assess the expression levels of individual genes, PCR amplification was conducted with Premix TaqTM in a Gradient PCR Instrument (Eppendorf, Germany). ..

    Labeling:

    Article Title: Effects and Mechanisms of Cadmium on the Degradation of Microcystin-LR by Sphingopyxis sp. YF1
    Article Snippet: In this study, we investigated the effects of cadmium concentration on the degradation of microcystin-LR by Sphingopyxis sp. YF1, a highly efficient microcystins degrading bacterium.. Our results demonstrate that 0.. 25 mg/L cadmium decreased the degradation rate at 30 min, but had no significant effect on the biodegradation of MC-LR after 60 min; however, 4 mg/L cadmium significantly reduced the biodegradation rate of MC-LR throught the time points tested.

    Liquid Chromatography:

    Article Title: Effects and Mechanisms of Cadmium on the Degradation of Microcystin-LR by Sphingopyxis sp. YF1
    Article Snippet: In this study, we investigated the effects of cadmium concentration on the degradation of microcystin-LR by Sphingopyxis sp. YF1, a highly efficient microcystins degrading bacterium.. Our results demonstrate that 0.. 25 mg/L cadmium decreased the degradation rate at 30 min, but had no significant effect on the biodegradation of MC-LR after 60 min; however, 4 mg/L cadmium significantly reduced the biodegradation rate of MC-LR throught the time points tested.

    Microscopy:

    Article Title: Effects and Mechanisms of Cadmium on the Degradation of Microcystin-LR by Sphingopyxis sp. YF1
    Article Snippet: In this study, we investigated the effects of cadmium concentration on the degradation of microcystin-LR by Sphingopyxis sp. YF1, a highly efficient microcystins degrading bacterium.. Our results demonstrate that 0.. 25 mg/L cadmium decreased the degradation rate at 30 min, but had no significant effect on the biodegradation of MC-LR after 60 min; however, 4 mg/L cadmium significantly reduced the biodegradation rate of MC-LR throught the time points tested.

    Transmission Assay:

    Article Title: Effects and Mechanisms of Cadmium on the Degradation of Microcystin-LR by Sphingopyxis sp. YF1
    Article Snippet: In this study, we investigated the effects of cadmium concentration on the degradation of microcystin-LR by Sphingopyxis sp. YF1, a highly efficient microcystins degrading bacterium.. Our results demonstrate that 0.. 25 mg/L cadmium decreased the degradation rate at 30 min, but had no significant effect on the biodegradation of MC-LR after 60 min; however, 4 mg/L cadmium significantly reduced the biodegradation rate of MC-LR throught the time points tested.



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